Abstract：In this study, sprawl cucumber（Cucumis sativus L.）129 and dwarf cucumber D0462 were taken as experimental materials. The contents of GA, IAA, ZR and ABA of apical meristems, leaves and roots were investigated, respectively by enzyme-linked immunosorbent assay（ELISA）. The results showed that the contents of GA in D0462 were very significantly lower than that of 129. However, the contents of IAA in D0462 were higher than that of 129 in early growing stages, but significantly lower in mid and late growing stages. The contents of ZR and ABA were very significantly higher than that of 129 in different growing stages. In addition, the ratio of GA/ABA，IAA/ABA and（IAA+GA+ZR）/ABA in D0462 were very significantly lower, but the ratio of ZR/GA were very significantly higher than that of 129. These results indicated that the defect of GA might lead to the dwarf of D0462, and the dwarf was also regulated by multifarious hormones.
