Abstract:The potato cultivar Longshu No.3,which is sensitive to Agrobacterium tumefaciens,was used as the transformation receptor.The regeneration and transformation systems via Agrobacterium tumefaciens were studied.For stem callus induction,the optimal medium was MS+6-BA 2.5 mg·L-1+NAA 0.5 mg·L-1+GA3 5.0 mg·L-1+2,4-D 1.0 mg·L-1,and the optimal medium for differentiation was MS+6-BA 3.5 mg·L-1+NAA 1.0 mg·L-1+GA3 10.0 mg·L-1.For mini-tuber chip,the optimal regeneration medium was MS+ZT 2.0 mg·L-1+IAA 1.0 mg·L-1.The ...
收稿日期: 2007-01-04
引用本文:
奇恩放;张金文;王一航. 反义AcInv基因导入马铃薯遗传转化体系的优势[J]. 中国蔬菜, 2007, 1(8): 10-14.
Qi Enfang;Zhang Jinwen;Wang Yihang. The Efficient Protocol for Transforming Antisense AcInv Genen into Potato. , 2007, 1(8): 10-14.