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| Identification and Analysis of NBS-LRR Family Genes in Dioscorea opposita Based on Transcriptome Sequencing |
| LI Shunpeng1，LI Chaochuang1，2，WANG Lanning1，ZHANG Hao1，2，WANG Ruifei1，2，LI Mingjun1，3，YANG Qingxiang1，2* |
| 1 College of Life Sciences，Henan Normal University，Xinxiang 453007，Henan，China；2 Henan International Joint Laboratory of Agricultural Microbia l Ecology and Technology，Xinxiang 453007，Henan，China；3 Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs of Henan Province，Xinxiang 453007，Henan，China |
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Abstract To explore the role of NBS-LRR resistance genes in response to different fungal infections in ‘Tiegun’ yam，NBS-L RR genes DoRGA2 and DoRGA4 were amplified from the genome of ‘Tiegun’ yam by homologous cloning．RT-qPCR analysis revealed that the infection of the black spot disease pathogenic fungus Alternaria alternata and the endophytic fungus Penicillium halotolerans could induce the up-regulated expression of DoRGA2 and DoRGA4 in different degrees．Meanwhile，RNA-seq was performed to analyze the NBS-LRR family genes of ‘Tiegun’ callus infected by A. alternata and P. halotolerans，the results showed that a total of 47 NBS-LRR family genes were identified，including 5 types of N，NL，CN，CNL and TN，with 24，15，3，3 and 1 gene，respectively．Furthermore，different subfamilies of NBS-LRR proteins had diff erent evolutionary paths，and these proteins were mainly acidic proteins with hydrophilic properties，and were mainly located in the cell membrane or cytoplasm．In addition，among these NBS-LRR family genes，the expressions of TRINITY_DN9146_c0_g1，TRINITY_DN8009_c1_g1，TRINITY_DN49830_c0_g1 and TRINITY_DN39475_c0_g1 were significantly up-regulated after inoculation with A. alternata，while TRINITY_DN18700_c0_g3 was significantly up-regulated after infection with P. halotolerans．
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Received: 15 March 2024
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