Taking broccoli(Brassica oleracea L.var.italica Plenck)variety‘Qingnong 70’and‘Qingnong 80’as experimental materials,this paper studies on plant regeneration factors of microspore embryoids.The results showed that the embryoids age(30-35 days)was optimal to plant regeneration,and the regeneration frequency exceeded 75 %,while it decreased significantly when the embryoids age was over 40 days.The regeneration frequency reached the highest(up to 60 %-70 %)when agar in the medium was 1.2 %.Most of embryoids could not produce plantlets directly,subculture was needed,and this treatment helped to differentiate much more plantlets.The optimal media for subculture was MS+3 % sucrose+1.0 % agar.About half of the regenerated plants were haploid,30 %-35 % plants were double haploid,and the rest were a few tetraploid or chimeras.