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Influence of Seed Disinfection Methods on Aseptic Welsh Onion Tissue Culture |
DONG Fei1,CHEN Yun-qi2*,LIU Shi-qi1,GAO Li-min2,WANG Chuan-zeng1 |
1College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018,Shandong,China|2Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province,Vegetable Research Institute,Shandong Academy of Agricultural Sciences,National Improvement Center for Vegetable,Jinan 250100,Shandong,China |
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Abstract Used sodium hypochlorite and mercuric chloride 2 common disinfectants to conduct disinfection treatment for welsh onion(Allium fistulosum L.)and studied the effect of Tween-20 onion seed disinfection.The results showed that 40 min soak in 2% sodium hypochlorite,and adding Tween-20 before and after disinfection was the highest in disinfection effect for welsh onion seeds,could significantly improve seed germination rate,germination energy and reduced pollution rate.
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Received: 01 June 2011
Published: 14 July 2011
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