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| Determination of Silencing Suppressor of Tomato Yellow Leaf Curl Virus by Agroinfiltration |
| ZHANG Yu-yang1，YEDIDYA Gafni2，LI Han-xia1* |
| 1Key Laboratory of Horticultural Plant Biology，Ministry of Eduction，Huazhong Agricultural University，Wuhan 430070，Hubei，China|2Institute of Plant Sciences，Agricultural Research Organization of Israel，Bet Dagan 50250，Israel |
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Abstract We described the utilization of agroinfiltration to screen and verify the silencing suppressor，V2，of Tomato yellow leaf curl virus（TYLCV）．The target gene together with the GFP gene was transiently co-expressed in leaves of Nicotiana benthamiana by agroinfiltrations．In all tests of agroinfiltrations，the GFP showed high expression at 2 days post inoculation（dpi），showing the infiltration and expression was successful．On the leaves co-infected with the BV1 gene of Watermelon chlorotic stunt virus or the empty vector pBIN，the GFP fluorescence almost disappeared at 7 dpi due to silencing mechanism of the host plant．The leaves co-infected with V2，showed continuously high expression of GFP，indicating that V2 is the silencing suppressor of TYLCV．Agroinfiltration can be utilized as potent tool for screening the silencing suppressor of plant virus．
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Received: 02 February 2012
Published: 03 March 2012
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