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| Inducement and Identification of Yellow Flesh Tetraploid Watermelon |
| ZHANG Na，SUN Yu-hong*，DING Ming，ZENG Hong-xia，SHI Xian-feng，REN Jian，LI Yu-hua，DU Nian-hua，TANG Mi |
| Wuhan Agricultural and Science Institute，Wuhan 430345，Hubei，China |
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Abstract We induced tetraploid watermelon by treating the seedling growingpoints of yellow flesh diploid watermelon with 0.3% and 0.4% solution of colchicines．Autotetraploid plants were selected and identified on morphology，flow cytometry，hybridization and quality．After 3 successive quarters cultivation and observation，we gained a stable tetraploid．The results showed that the most effective treatment was extirpating interior-leaf at the 8th day after sowing，then treating the seedling growing points with 0.3% colchicine for 6 days．The survival rate of seedlings could reach over 80% and the induction frequency of tetraploid watermelon could be stabled above 16%．The treatment can improve the accuracy of selection high-quality tetraploid material combined with other identification methods．
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Received: 17 August 2011
Published: 23 September 2011
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