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| Effect of Different Explants Treatments on in vitro Plant Regeneration of Pepper（Capsicum annuum L.） |
| CAO Ya-cong，ZHANG Zheng-hai，WANG Li-hao，MAO Sheng-li，ZHANG Bao-xi* |
| Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract The hypocotyl segments of pepper seedlings，seedlings without capitals，seedlings decapitated after wounded by puncturing the hypocotyls，the half seeds consisting of the proximal part of the hypocotyls and the radicles，and the half seeds consisting of the cotyledons，the apical part of the hypocotyls and the embryos of 5 inbred lines（0510，0516 and 7013 were sweet pepper，0818 and 0819 were hot pepper）of pepper（Capsicum annuum L．）were used as explants for regeneration．MS medium with or without PGSs（plant growth substances）was used for culturing．There was not any regenerant obtained from explants cultured in the medium with PGSs，while some regenerate shoots emerged from explants cultured on the PGSs-free medium，of which the average regenerate rate of seedlings decapitated when the cotyledons did not exposure yet（about 7 days after sowing）and seedlings decapitated at the curved hypocotyl stage（about 9 days after sowing）was 0.33%，seedlings decapitated after hypocotyls punctured while the hypocotyls just escaped from curved stage（seedlings were wounded by puncturing the hypocotyls after sowing about 10-12 days，and decapitated after another 12 days）was 2.43%，seedlings decapitated after hypocotyls punctured at the curved hypocotyl stage（seedlings were wounded by puncturing the hypocotyls after sowing about 9 days，and decapitated after another 12 days）was 12.08%，the half seeds consisting of the proximal part of the hypocotyls and the radicles（intact seeds were pre-cultured on wet filter paper for 3 days，and then cut into 2 halves for culture）was 17.33%．The regenerate rate of hot pepper 0818 was 46.67%，when the half seeds consisting of the proximal part of the hypocotyls and the radicles were used as explants and cultured on the PGSs-free medium，and 0819 was 33.33%．Moreover the regenerate buds could easily elongate．This method could be used for pepper transgenic study，due to its simple procedure，high regenerate rate and speediness of regeneration．
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Received: 12 December 2011
Published: 20 February 2012
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