|
|
Distribution of S-haplotypes in Cabbage F1 Hybrid |
MIAO Wen-wen,TIAN Lei,ZHUANG Mu*,LIU Yu-mei,YANG Li-mei,ZHANG Yang-yong,
FANG Zhi-yuan |
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
|
|
Abstract DNA fragments corresponding to kinase domain of S locus kinase( SRK) gene were
amplified by PCR with 3 pairs of specific primers in all of the 23 cabbage(Brassica oleracea L. var.
capitata L.) F1 hybrids.The S haplotypes of cabbage F1 hybrid were preliminary identified via DNA
sequencing and Blast analysis.The results indicated that 11 S haplotypes were found in cabbage F1
hybrid,including class Ⅰ haplotypes,S7,S16,S28,S35,S45,S57,S68 and an unknown one
named Sn,and class Ⅱ haplotypes S2,S5 and S15.The highest frequency of S haplotyes was S5 with
19.6% and followed by S28 with 17.4% in this study.Compared over 50 S haplotypes in Brassica,the
11 S haplotypes in cabbage F1 hybrid suggests that it is very necessary for exploiting more S haplotypes
in cabbage breeding programs.
|
Received: 06 March 2013
Published: 09 April 2013
|
|
|
|
|
|
|
|