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Defense Response of Hemolymph in Bemisia tabaci to Beauveria bessiana |
REN Jie1,HAN Xue-mei2,LIU Zhao3,LEI Zhong-ren4* |
1College of Plant Science, Tarim University, Alar 843300, Xinjiang, China; 2College of Life Sciences, Nanyang Normal University, Nanyang 473061, Henan, China; 3College of Plant Protection, Southwest University,Chongqing 400716, China; 4State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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Abstract To identify the defense mechanism of Bemisia tabaci hemolymph against Beauveria bassiana,cellular immune responses of the infected 3rd instar nymph was investigated with optics microscope and Giemsa stain.The results showed that the invasion of Beauveria bassiana could bring about changes in the total numbers of host hemocytes.After 36 hours inoculation,the blastospores appeared in B. tabaci and the total numbers of host hemocytes were increased.At the same time,the blastospores were adsorbed to the surface of plamatocyte.60 hours after inoculation,the hyphal bodies were absorbed to the surface of granulocytes.More hyphal bodies were observed to the surface of granulocytes 96 hours after inoculation,and the insect body color had appeared the symptoms of infection,which means that the B. bassiana has broken the defense of B. tabaci.B. tabaci can only inhibit the invasion of B. basiana temporarily.
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Received: 18 March 2013
Published: 15 April 2013
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