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Clone and Expression Analysis of Six Auxin-related Genes in Brassica oleracea L.var. capitata L. |
PU Quan-ming1,GAO Qi-guo1*,ZHANG Lin-cheng1,SHI Song-mei1,LIU Xiao-huan1,ZHANG Ying1,BI Yun-long2,REN Xue-song1,LIU Yu-dong1,ZHU Li-quan2 |
(1Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University, Chongqing 400716,China;2College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China) |
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Abstract Auxin plays an important role in regulating the process of Brassica oleracea L. var. capitata L.leaf curl.In order to find out the auxin relevant genes involved in cabbage leaf curling,we analyzed transcriptome of cabbage leaf at rosette and heading stages and identified 6 significantly differentially expressed auxin-related genes,including regulation of homeostasis genes(BoILL6,BoASA1),controlling polar transport genes(BoSF21,BoPIN4)and involving in signal transduction genes(BoARF8,BoGH3.5).The full-length cDNA sequences of 6 genes were obtained,respectively using homologous clone method.The protein structure prediction revealed that 6 genes contained auxin-related domains.Phylogenetic analysis though MEGA 5.1 comparison displayed that these genes had the closest genetic relationship with correspondence genes of Brassica rapa,and Arabidopsis thaliana followed. The real-time Fluorescence quantitative PCR analysis suggested that the expression level of BoILL6,BoASA1 and BoSF21 showed significantly different trend.The expression level of these 3 genes in leaf during heading stage were 12 folds more than that in the leaf at rosette stage.The trend was consistent with the
results of transcriptome analysis.The up-regulated expression of these 3 genes in the leaf during heading stage maybe lead to enhance the auxin concentration and change the auxin polar contribution in the leaves,and finally lead to format of the leaf curl.
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Received: 26 August 2015
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