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Identification and Preliminary Application of Antagonistic Strains Against Potato Fungal Diseases |
YANG Fan 1,2,ZHANG Fei-yan2,SUN Jin-chong2,LIU Shuang3,LIU Hong-wei2,ZHANG Li-ping 2*,HU Bin 1* |
1Hebei University of Technology,Tianjin 300130,China;2Institute of Biology,Hebei Academy of Science,Shijiazhuang
050081,Hebei,China;3Hebei Animal Husbandry Station,Shijiazhuang 071000,Hebei,China |
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Abstract Aiming at the issues of serious occurrence of potato fungal diseases,mixed occurrence of
various diseases,lacking effective control methods,this paper classified and identified highly effective
antagonistic strain BA-26 screened out at earlier stage,studied the stability of its extracellular metabolites,
and clarified its role on preventing disease and promoting growth.BA-26 strain was identified as Bacillus
amyloliquefaciens,through analyzing morphologic characteristics,physiological and biochemical properties,
and 16S rDNA sequence analysis.This strain showed strong antagonistic effects on 8 potato pathogenic
fungus,and its inhibition ratio against Alternaria solani,Fusarium solani and Fusarium avenaceum was
over 80%.After treating the extracellular metabolites by 100 ℃ for 30 min,UV irradiation for 8 hours,pH
2-10 acid-base,and protease for an hour,its relative antibacterial activities were all maintained above 80%.
Laboratory test of bacteriostatic activity showed that the inhibition ratio of BA-26 filtrate against Fusarium
solani was 91.3%.In pot experiment,the potato root length,fibrous root number,plant height and
chlorophyll content in leaves were significantly increased by 32.07%,111.94%,48.0% and 19.6%,
respectively by applying BA-26 fermentation broth with 20 mL concentration of 4.0×109 cfu·mL-1 per pot.
The results indicated that B. amyloliquefaciens BA-26 had broad bacteriostasis spectrum,strong stability of
extracellular metabolites,and good disease prevention and growth promotion effect,having great potential for
developing biocontrol agent exclusively for potato.
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Received: 18 February 2019
Published: 04 April 2019
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