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Cloning of Heat Shock Protein Related Gene CmHSP70-5 and Expression Analysis under Heat Stress in Cucurbita moschata |
HU Yan-ping 1,ZHOU Yang 2,YANG Chun 3,LIAO Dao-long 1,ZHU Bai-bi 1,WANG Min 1,HUANG Wen-feng 1,ZHANG Wen 1,YUN Tian-hai 1* |
1 Institute of Vegetables,Hainan Academy of Agricultural Sciences,Hainan Key Laboratory of Vegetable Biology,Hainan
Vegetable Breeding Engineering Technology Research Center,Haikou 571100,Hainan,China;2 College of Horticulture,
Hainan University,Haikou 570228,Hainan,China;3 Hainan Academy of Agricultural Sciences,Haikou 571100,Hainan,
China |
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Abstract A heat shock protein gene,naming CmHSP70-5,was isolated from pumpkin cDNA by
polymerase chain reaction (PCR) method based on pumpkin genome sequence.Phylogenetic relationship
of HSP70-5 and its homology were analyzed using bioinformation method.The expression quantity of
CmHSP70-5 under heat stress was studied by real-time PCR.The results showed that CmHSP70-5 gene was 1
953 bp,and its coding CmHSP70-5 protein had the closest relative with Arabidopsis AtHSP70-5.CmHSP70-5
gene had a high expression level in roots than in stems and leaves under normal conditions.Real-time PCR
results indicated that under heat stress,the expression of CmHSP70-5 gene in roots,stems and leaves were all
up-regulated and could reach the maximum quantity 3 hours after treatment,indicating that the expression of
CmHSP70-5 gene was related to heat stress in Cucurbita moschata.
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Received: 15 February 2019
Published: 15 May 2019
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