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Analysis on ToCV and TYLCV Co-infection Ratio and Sequence Phylogeny in 6 Provinces of China |
HU Rong1,FENG Li-xiao1,ZHANG Yu1,ZHANG Song-bai1,2,ZHENG De-yong1,2,LIU Yong1,2* |
1Longping Branch of Graduate School,Hunan University,Changsha 410125,Hunan,China;2Plant Protection Institute,Hunan Academy of Agricultural Science,Changsha 410125,Hunan,China |
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Abstract From 2017-2019,in major tomato producing areas of 6 Chinese provinces,tomato samples suspected to be infected by tomato chlorosis virus(ToCV) and(or) tomato yellow leaf curl virus(TYLCV)were collected.This paper conducted RT-PCR or PCR by specific primers,then carried out phylogenetic analysis on PCR products conforming to expected band size after sequencing.Results showed that the recombination co-infection ratio of ToCV and TYLCV from 6 Chinese provinces was 25.29%,among them those of 2 virus from Shandong Province was the highest-46.43%.Results of sequences analysis indicated that the homology of measured ToCV CP gene sequence with reported corresponding sequence was over 98.30%.There were no obvious genetic differentiation in ToCV CP gene sequences.The homology of partial sequence of TYLCV genome with published corresponding sequence was over 96.00%,and no distinct genetic differentiation was occurred.
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Received: 29 October 2019
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