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Genetic Research and Gene Mapping of The Stalk Glossy Trait in Pakchoi |
HU Yang1,SONG Liping2,WANG Aihua2*,LIANG Hongyan1,QIU Wenbing1,CAI Meizhong1,CUI Xiaopei1,GAO Changbin2,ZHOU Guolin2,LI Ping1* |
1Jingzhou Academy of Agricultural Science,Jingzhou 434007,Hubei,China;2Wuhan Academy of Agricultural Science,Wuhan 430070,Hubei,China |
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Abstract Using the stalk glossy inbred line Q161 and stalk waxy inbred line Q195 as parent,this paper constructed the F1,F2,BC1P1 and BC1P2 colony and analyzed the genetic law for stalk glossy trait in pakchoi.The result showed that the stalk glossy trait of pakchoi was controlled by a pair of recessive genes.The F2 bulked segregant population were detected by the 30× BSA-seq analysis and the parents were detected by the 20× genomic resequencing analysis.The stalk glossy gene was mapped between 44.1-45.0 Mb interval of A09 chromosome.The resequencing data of the parents provided us with InDel markers.The stalk glossy gene was mapped between marker Br119 and Br116 on the A09 chromosome.The physical distance between these 2 markers was 380 kb.
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Received: 12 August 2020
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