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Evaluation on Resistance of Different Chinese Cabbage Varieties to Clubroot Pathogens Occurred in Different Regions |
MA Xiaochao,ZHANG Hui,ZHANG Shujiang,LI Fei,ZHANG Shifan,LI Guoliang,LIU Xitong,SUN Rifei* |
Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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Abstract In order to establish a rapid and stable technique for identifying Chinese cabbage resistance to clubroot at seedling stage,and explore the resistance performance of commercial Chinese cabbage varieties to clubroot pathogens from different sources,this experiment took the highly susceptible variety of Chinese cabbage‘Juxin’as test material,and clubroot pathogen from Beijing Shunyi as inoculation bacteria source,compared and optimized the inoculation conditions of injection inoculation method and root-dip inoculation method.Adopting the optimized root-dip inoculation method,this experiment also carried out artificial inoculated resistance identification on 15 Chinese cabbage varieties resistant to clubroot with clubroot pathogen from Henan Nanyang,Beijing Shunyi,and Chongqing Wulong as bacteria source.The results showed that when using the injection inoculation method,the injection volume over 2 mL had significant effect in comparison with injection volume of 1 mL,and when using the root-dip inoculation method,soaking root system over 5 min had the best inoculation effect.Different cabbage varieties showed inconsistent resistance performance to bacterial sources from 3 regions,among them‘CR Huimin’‘CR Limin’and‘Degao CR117’all had stronger resistance performance to clubroot pathogens from the 3 regions.While,‘CR Gaolengdi’‘CR Genbao’‘CR Jinbaili’‘Degao CR1138’‘Genwang’ ‘Wanqingkezhonghuang’showed vertical resistance.
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