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| Studies on Pathogenicity and Isolate Identification of Pathogen Causing Chinese Cabbage Black Spot in Qingdao Region |
| SONG Yumeng，LIU Wenjie，WANG Min，YUAN Zhonglin，LUO Lan* |
| （Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management，College of Plant Health and Medicine，Qingdao Agricultural University，Qingdao 266109，Shandong，China） |
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Abstract In order to clarify the kind of pathogen causing Chinese cabbage black spot in Qingdao region of Shandong Province，this experiment collected 18 accessions of sickened Chinese cabbage samples from Qingdao City Jiaozhou，Laixi and Jimo，in 2019—2020；isolated pathogenic fungi by tissue separation method；conducted classification and identification through morphological observation and molecular biological techniques；and carried out pathogenicity test according to Koch’s rule．The results showed that 21 strains were isolated from the sickened Chinese cabbage samples collected from Qingdao region，belonging to Alternaria brassicae，A. japonica and A. alternate，separately．Among them，A. alternate was the dominant pathogen with detection rate of 76.2%．But A. brassica was the most virulent with a disease index of 48.15．
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Received: 07 November 2022
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