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| Differential Analysis of Metabolites in Wax Powder on Surface of Broccoli Florets Based on Non-targeted Metabolomics |
| TAO Meiqi，PAN Yongfei，SHAN Xi，DAI Zhongliang，ZHANG Zhenchao* |
| Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province，Jurong 212400，Jiangsu，China |
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Abstract In order to reveal the composition difference of the wax powder metabolites on the surface of the broccoli inflorescence，taking the wax powder deficient mutant and the wild type of broccoli as test materials，based on the non-targeted metabolomics of gas chromatography tandem time-of-flight mass spectrometry（GC-TOF-MS），the main metabolic pathways and their products of the broccoli wax powder deficient mutant and the wild type were deeply analyzed，and the significantly different metabolites were screened by using univariate and multivariate variable statistical analysis and database annotation．The results showed that a total of 24 significantly different metabolites were detected in the wax powder of broccoli buds，including 14 kinds of amino acids，4 kinds of organic acids，1 kind of fatty acid，1 kind of amine，1 kind of sugar，and 3 kinds of other secondary metabolites．Among them，there were 18 kinds of down-regulated，including 13 kinds of amino acids．Through comprehensive enrichment analysis and topological analysis，11 important metabolic pathways with prominent significance were obtained，among which the most significantly different metabolic pathway was the biosynthesis of amino acids，followed by the biosynthesis of secondary metabolites；and the most important one was tryptophan metabolism．Therefore，it is speculated that the lack of broccoli wax powder can regulate the content such as amino acids and organic acids and other secondary metabolites in the broccoli buds by regulating the pathways such as tryptophan metabolism，the biosynthesis of secondary metabolites，and the biosynthesis of amino acids，then regulate the growth of the inflorescence，the nutritional quality，and the cold resistance．
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Received: 01 November 2023
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