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| Gene Mining of Broccoli in Response to Drought Stress |
| GAO Xu1，HE Daogen1*，TAN Guoyin1，ZHU Changzhi1，ZHANG Hongyun2，YANG Mingfan3 |
| 1Taizhou Academy of Agricultural Sciences，Taizhou 318014，Zhejiang，China；2Zhejiang Wuwangnong Seeds Sharcholding Co.，Ltd.，Hangzhou 310020，Zhejiang，China；3Taizhou Vocational College of Science & Technology，Taizhou 318020，Zhejiang，China |
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Abstract To mining the key genes of drought resistant genes in broccoli，the effect of biomass and antioxidant enzyme activity of broccoli seedlings were analyzed，with‘Tailv No.3’for material，simulated drought stress by PEG，and differentially expressed genes related to antioxidant enzymes screened by transcriptome sequencing，then expression level analyzed based on real time fl uorescence quantitative PCR．The results showed that the aboveground biomass accumulation of broccoli seedlings was signifi cantly inhibited and the growth of underground parts was promoted，as well as the activities of POD，SOD，CAT，APX and GPX increased．Transcriptome sequencing analysis showed that 6 513 genes diff erentially expressed was detected in broccoli seedlings under drought stress，of which 3 129 genes were up-regulated and 3 384 genes were down-regulated．45 differentially expressed genes related to antioxidant enzymes were screened，then one gene related to POD，SOD，CAT，APX and GPX was screened with the highest up-regulation amplitude by analysis of relative expression quantity，respectively．These genes may be key genes for drought resistance in broccoli，which provides a scientifi c basis to research drought resistance mechanism．
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Received: 18 December 2023
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