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Effi cient Regeneration System for Brassica olerecea Based on Epicotyl Explants |
HAN Xichao1,2,ZHANG Xiaojian2,CAI Xu2,LIANG Jianli2,WANG Xiaowu2,ZHU Keming1*,WU Jian2* |
1College of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,State Key Laboratory of Vegetable Biobreeding,Beijing 100081,China |
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Abstract The hypocotyl is a commonly used explant for genetic transformation of cabbage(Brassica oleracea var. capitata),however,the problems of genotype-dependent and low regeneration efficiency are the main obstacles at present.This study explores the construction of a genetic transformation system using the epicotyl of cabbag e as an explant,with 9 cabbage varieties as test materials.The regeneration effi ciency after Agrobacterium infection was compared between the hypocotyl and epicotyl as two explants.The results show that 5 cabbage varieties had elongated epicotyls.After Agrobacterium inoculation,all 5 varieties with epicotyls as explant induced adventitious shoots with regeneration frequencies ranging from 32.4% to 78.4%.In contrast,only 3 varieties induced of adventitious buds with hypocotyl as explant,with the highest regeneration frequency of only 2.7%.This shows that the epicotyl is an ideal explant for overcoming genotypic dependence in B. olerecea transformation,and provides an effi cient approach to obtain regenerated B. oleracea plants.
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Received: 09 April 2024
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