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| Screening and Analysis of Genes Related to Growth and Expansion of Yam（Dioscorea alata L.）Tubers |
| LIANG Renfan1，2，HUANG Hao2，ZHOU Shengmao2，WANG Aiqin1，ZHAN Jie1，WEN Junli2，HE Longfei1* |
| 1College of Agronomy，Guangxi University，Nanning 530004，Guangxi，China；2Guangxi Academy of Agricultural Sciences，Nanning 530007，Guangxi，China |
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Abstract To screen the genes related to the growth and expansion of yam tubers，bioinformatics methods were used to analyzed the suppression subtractive hybridization（SSH）libraries at different developmental stages of yam tubers in the early and middle stages．The results showed that the diff erentially expressed genes（DEGs）sequences in the early and middle stages of the development of yam tubers mainly involved nine categories，of which developmental regulation accounts for the largest proportion（27.1%），followed by cellular tissue structure（14.0%），material metabolism（11.2%），and biosynthesis（4.7%）．Preliminary screening has identified some genes，including F-box associated，carbonic anhydrase（CAs），nucleoside diphosphate kinase（NDPK），amino acid permease，glycosyl transferase，and alpha amylase．These genes may be related to cell proliferation，elongation，hormone metabolism，acid-base environment regulation，and functional metabolite regulation，and are involved in regulating tuber growth and expansion in yam．
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Received: 02 January 2024
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