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Construction of RNAi Expression Vector of Pectatelyase from Strawberry |
QIAN Chun, HE Qiao, ZHAI Jun-peng, SONG Bo, XIA Bei-bei, ZHANG Xing-guo, LIANG Guo-lu* |
College of Horticulture and Landscapal, Southwest University, Chongqing 400715, China |
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Abstract In order to enhance the firmness of strawberry(Fragaria ananassa Duch.)and extend the shelf life, this experiment constructed RNA interference(RNAi)expression vector using a 382 bp conservative fragment of pectate lyase. The target gene was successfully transferred into strawberry via Agrobacterium. Two postitive transformants were detected through PCR analysis.
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Received: 11 February 2010
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Corresponding Authors:
LIANG Guo-lu
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