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| Preliminary Identification of S Haplotypes of Two Self-incompatible Lines in Cabbage（Brassica oleracea var. capitata L.） |
| TIAN Lei, ZHUANG Mu*, LIU Yu-mei, YANG Li-mei, ZHANG Yang-yong, FANG Zhi-yuan |
| Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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Abstract According to the conserved sequence of S locus kinase（SRK）gene in cabbage（Brassica oleracea var. capitata L.）, a pair of SRK-specific primers was designed. The S haplotypes of 2 inbred lines in cabbage were identified by PCR amplification and DNA sequencing, combined with bio-informatics analysis. BLAST results indicated that the self-incompatible line 96-100 showed 91 % similarity in nucleotide sequence with SRK29 gene in cabbage, and 100 % similarity under 84 % query coverage of protein residue with S14 haplotype in cabbage, while the self-incompatible line Eyin165 exhibited 100 % similarity in nucleotide sequence with BoSRK28 gene in cabbage. This indicated that S haplotype of Eyin165 is S28.
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Received: 15 December 2010
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