Abstract: The change of nucleic acid content in broccoli Brassica oleracea L. var. italica Plenck during vernalization under temperatures in daytime/at night=（17.3±1）℃/（9.3±1）℃ was studied using cultivar‘Qingfengqinghuacai103’with 5 true leaves as experiment material. The results showed that after vernalization, the contents of DNA、RNA and nucleic acid, the ratio of RNA/DNA were higher than the control（CK）, which had not be vernalized, and these contents increased with the process of vernalization. Among them, the contents of RNA and total nucleic acid, and the ratio of RNA/DNA were rapidly increased before the vernalization, while the DNA content was swiftly increased at the end of vernalization.

Key words: Brassica oleracea L. var. italica Plenck, Vernalization, Nucleic acid, RNA, DNA

摘要： 以早熟青花菜品种清风青花菜103为试材，对5片真叶期的植株进行春化处理〔昼温/夜温=（17.3±1）℃/（9.3±1）℃〕，研究青花菜绿体春化过程中核酸含量的变化规律。结果表明：经绿体春化后，青花菜体内的DNA、RNA和总核酸含量以及RNA/DNA的比值均比未经过春化处理的对照高，且均随着春化的进程而升高；其中RNA、总核酸含量和RNA/DNA的比值在春化完成前均开始迅速增加，而DNA含量则在春化作用结束时才迅速增加。