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| SSR Inheritance Analysis and Screening for Linked Marker of Powdery Mildew Resistance in Cucumber（Cucumis sativus L.） |
| NIE Jing-tao1, PAN Jun- song2, HE Huan-le2, SI Long-ting1*, CAI Run2* |
| 1School of Horticulture, Shenyang Agricultural University, Shenyang 110161, Liaoning, China; 2School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China |
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Abstract In order to accelerate molecular marker assisted breeding process of powdery mildew resistance in cucumber（Cucumis sativus L.）, in this paper, high susceptible cucumber inbred line M12, abd high resistant inbred line M3 to powdery mildew were taken as parent and their hybrid, F2 populations and BC1 populations were used as experimental materials. We identified the seedlings inoculated with powdery mildew fungus and probed into the genetic regulation of powdery mildew resistance in cucumber. Combing with BSA method and SSR technology, SSR markers linked to the major resistant gene of powdery mildew in cucumber was obtained. The results showed that the resistance to powdery mildew was mainly controlled by a single recessive gene. By analyzing F2 single plant with SSR technique, a marker SSR15592 linked to the resistant gene was identified. The genetic distance between this marker and resistant gene was 7.62 cM.
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Received: 29 October 2010
Published: 16 March 2011
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