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| Agrobacterium Tumefaciens Mediated Transformation of Cabbage Fusar- ium Wilt Pathogen |
| LI Er-feng，WANG Dian-dong，YANG Yu-hong，XIE Bing-yan* |
| Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract Agrobacterium tumefaciens mediated transformation（ATMT）was applied in this research．Agrobacterium tumefaciens，which carried plasmid PCH-sGFP harboring the green fluorescent protein（gfp）gene and hygromycin phosphotransferase gene（hph），was used to transform Fusarium oxysporum f.sp. conglutinans race 1 and 2．The transformants were randomly selected to confirm．The results indicated that transformants were integrated with exogenous T-DNA in genomes successfully，and gfp gene was stable after 6 successive subcultures．The growth rates analysis and pathogenicity showed that there were no significant differences between wild isolates and transformants on growth rates and pathogenicity．So transformant can be used as a useful tool for further function research，such as histopathology and pathogenesis.
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Received: 24 March 2011
Published: 02 June 2011
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