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| Orange Morphological Marker’s Applications in Transferring Male Sterile Lines of Orange Head Chinese Cabbage with Anti-clubroot Genes |
| GENG Xin-cui，SHEN Xiang-qun*，LI Qian，CHEN Yong-hao，GUO Yan-feng |
| Department of Horticulture，Shenyang Agricultural University，Shenyang 110866，Liaoning，China |
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Abstract The flower color and cotyledon color could be as morphological marker traits used in transferring orange leaf head Chinese cabbage〔Brassica campestris L.ssp. pekinensis（Lour）Olsson〕for their heredity agreed on that of leaf head color of orange leaf head Chinese cabbage．This color was controlled by a pair of recessive alleles．In this paper，the methods of leaf head color，cotyledon color and flower color were compared during the course of transferring the male sterile lines of orange leaf head Chinese cabbage with anti-clubroot genes under different conditions．When cotyledons were just unfolded，orange leaf head plants were found and picked out with the help of cotyledon color method in situations of seed vernalization or non-seed vernalization survey．Compared with other 2 methods，when screening clubroot-resistant plants，the amount of plants needed to identify were the least by means of cotyledon color method，so did the amount of Plasmodiophora brassicae．Therefore，for it had a wider range of applications and less work intensity，the method of cotyledon color were optimal among these 3 methods．It might be used in breeding resistant varieties or male sterile lines of orange leaf head Chinese cabbage．
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Received: 14 December 2011
Published: 07 February 2012
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