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| CAPS Markers of BoCBF1 and BoCBF2 Genes in Cabbage and Their Contribution to Cold-tolerance |
| JIN Zhe，ZHANG Yang-yong*，FANG Zhi-yuan，LIU Yu-mei，YANG Li-mei，ZHUANG Mu，LIU Ji-sheng，SUN Pei-tian |
| Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract Two cabbage（Brassica oleracea var. capitata L.）inbred lines differing in cold-tolerance were used to analyze the effect of CBF gene variation on cold-tolerance．Based on Arabidopsis CBF1 gene sequence and the scaffold information of cabbage genome，the full-length coding domain sequence of cabbage BoCBF1 and BoCBF2 genes were obtained．PCR products were sequenced directly to discover 4 single nucleotide polymorphisms （SNP）between the inbred lines 341 and 21-3 for BoCBF1 gene and 16 SNP and one insertion-deletion （InDel）for BoCBF2 gene．In order to rapidly and reliably detect the sequence variation of BoCBF1 and BoCBF2 genes，2 CAPS markers were successfully converted and named as BoCBF1-TaqαⅠ，BoCBF2-BstUⅠ．The CAPS markers were used to screen inbred lines 341，21-3 and their F1，F2 population．The analysis showed that the variation of 2 genes very significantly correlated with cold-tolerance，so the CAPS markers can be used for marker-assisted selection（MAS）in cabbage cold-tolerance breeding．
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Received: 05 April 2012
Published: 02 May 2012
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