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Studies on Pumpkin Genetic Transformation Mediated by Ovary Injection |
ZHANG Yan-chao,GE Yu,YU Yang,XU Wen-long,BAI Yu-jiao,CUI Chong-shi,QU Shu-ping* |
Horticulture College of Northeast Agricultural University,Harbin 150030,Heilongjiang,China |
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Abstract Taking pumpkin(Cucurbita moschata Duch.?ex Poir.)variety‘Yinhui No.?1’as receptor,aiiA gene was transferred into 148 pumpkin ovaries by injection.115 fruits were obtained with 77.7% seed setting percentage.Then 56 fruits were randomly selected,screened by 0.2% Basta aqueous solution and PCR identification.Finally,7 positive plants were all from one single fruit and all of them showed the hybridization signals by PCR-Southern Blot identification.The result indicated that the aiiA gene had been integrated into the genomic DNA of pumpkin‘Yinhui No.?1’with a converting rate of 0.25% and the converting rate per fruit was 6.36%.
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Received: 22 February 2012
Published: 05 April 2012
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