|
|
|
| Establishment of High-frequency Regeneration System from Cotyledons with Petiole in DH Lines of Broccoli |
| SHENG Xiao-guang，GU Hong-hui*，YU Hui-fang，WANG Jian-sheng，ZHAO Zhen-qing |
| Institute of Vegetables，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，Zhejiang，China |
|
|
|
|
Abstract The high frequency of shoot regeneration from cotyledons with petiole in broccoli（Brassica oleracea L.?var.?italica Plenck）was studied and the effect of the different genotypes，different mass concentration plant growth regulator，different age of explants and different concentration AgNO3 on regeneration frequency of adventitious buds were studied．The results showed that 2 DH lines，3-6 and 3-7，obtained high shoot differentiation rate（96.9% and 97.8%，respectively），after the explants were pre-cultured for 8 days，then placed in the MS medium supplemented with 0.02 mg·L-1 NAA，1.0 mg·L-1 6-BA，4 mg·L-1 AgNO3，10 g·L-1 agar and 30 g·L-1 sucrose．The frequence of shoot regeneration was 15 and 14．The feasible rooting medium was MS + 0.05 mg·L-1 NAA +7 g·L-1 agar + 30 g·L-1 sucrose and the rooting rate reached to 100%．
|
|
Received: 04 July 2012
Published: 16 August 2012
|
|
|
|
|
|
|
|
|
