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Application of Different Culture Medium in Onion Tissue Culture |
PAN Mei-hong,CHEN Zhen-tai*,XUE Ping,YANG Hai-feng |
Lianyungang Academy of Agricultural Sciences,Liangyungang 222000,Jiangsu,China |
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Abstract In order to improve onion(Allium cepa L.)tissue culture technology,this study used different basal medium,added different growth regulators on different onion varieties.The results showed that the onion variety‘Zaochunhuang’in culture medium of MS+6 mg• L-1 6-BA+0.1 mg• L-1 NAA had the best proliferation effect.The onion variety‘Liancong No. 6’in culture medium of 3 g•L-1 Hyponex +6 mg•L-1 6-BA +0.1 mg•L-1 NAA had the best proliferation effect.In successive transfer culture,adding 0.25 mg•L-1 paclobutrazol could raise the proliferation rate.
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Received: 29 March 2012
Published: 24 July 2012
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