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Research Progress in Genetic Transformation of Brassica campestris L.ssp. chinensis(L.)Makino var.communis Tsen et Lee |
CHEN Ling-xian,CUI Li-jie,QIAN Zhong-ying,PAN Jing-xian,KAI Guo-yin* |
College of Life and Environment Sciences,Plant Germplasm Resources Research and Development Center,Shanghai Normal University,Shanghai 200234,China |
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Abstract Brassica campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee belongs to Brassica,Cruciferae and is an important leaf vegetable widely cultivated in Yangtze River basin and various southern provinces in China.It is urgently needed by production for cultivating varieties with disease-resistance,insect-resistance,stress-resistance and superior agronomic traits.Genetic transformation technology has provided a new way to solve problems in cultivating insect and disease resistant and heat tolerant varieties.This paper expounds the research progress made in regeneration system,genetic transformation methods and exogenous gene expression of Brassica campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee.Agro-bacterium mediated method has become the most commonly used method for genetic transformation of Brassica rapa for its low cost,simplicity,easy operation and high transformation efficiency.
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Received: 21 July 2012
Published: 10 October 2012
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