|
|
Changes of Endogenous Hormone Contents during in vitro Regeneration of Adventitious Bud of Cucumis melo L. Inbred Line |
ZHANG Hui-jun,LUAN Fei-shi*,WANG Xue-zheng,GAO Peng,NIU Ming-ming |
Horticulture College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China |
|
|
Abstract Taking cotyledonary nodes collected from melon(Cucumis melo L.)as experimental materials,this study tested the changes of hormones contents during in vitro regeneration of adventitious buds.The results indicated that the contents of ZR,IAA,ABA and GA3 culture were higher during the early stage of inoculation,and the ZR contents were higher at the stage of cell vigorous division and bud primordium formation,while the IAA contents were lower.Compared with lines with different regeneration capacity,the level of ZR/ABA of easy regeneration line was higher,while the IAA/ZR kept at a lower level,indicating adventitious bud formation from cotyledonary node of melon was closely associated with the contents of endogenous hormone.
|
Received: 03 March 2012
Published: 27 July 2012
|
|
|
|
|
|
|
|