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| Clone and Core Sequence Analysis on CP Gene of TYLCV Isolate fromTomato in Yangling |
| LI Yun-zhou，LIANG Yan*，WANG Qiao-li，LI Cui，CUI Xia，QIN Lei |
| College of Horticulture， Northwest A & F University， Yangling 712100， Shaanxi， China |
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Abstract Taking the tomato plants with TYLCV symptom obtained in Yangling tomato production
base and named TYLCV-Yl（GenBank accession：KC293824）as material，we cloned the full coat
protein gene（ CP） and the core part（tCP）of coat protein gene of the TYLCV-Yl isolate and analyzed
the sequence features and tCP evolutionary character．Multiple comparison of this virus full coat protein
sequence with other 18 TYLCV-CP nucleotide sequence and constructed evolutionary tree based on CP
gene nucleotide sequence using DNAMAN software．The results indicated that the tomato yellow leaf
curl virus isolation（ TYLCV-Yl）obtained in Yangling was confirmed as the resource of TYLCV-Yl．
We also acquired the full gene sequence of coat protein of TYLCV-Yl，which lay foundation for resistance
based on CP gene．The core sequence tCP is very conservative and its length is about 419 bp．
This core sequence coded 137 amino acids and has a trans-membra ne structure from 79 to 97 amino acid．
Based on phylogenetic tree constructed by CP gene，TYLCV from NCBI was divided into 3 subgroups：TYLCV subgroup Ⅰ，TYLCV subgroup Ⅱ and TYLCV subgroup Ⅲ．The isolate TYLCV-Yl was belonged
to subgroup Ⅰ.
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Received: 26 August 2012
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