Distribution of S-haplotypes in Cabbage F1 Hybrid

MIAO  Wen-Wen; TIAN  Lei; ZHUANG  Mu; LIU  Yu-Mei; YANG  Li-Mei; ZHANG  Yang-Yong; FANG  Zhi-Yuan

›› 2013, Vol. 1 ›› Issue (10): 23-28.

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Distribution of S-haplotypes in Cabbage F₁ Hybrid

MIAO Wen-wen，TIAN Lei，ZHUANG Mu*，LIU Yu-mei，YANG Li-mei，ZHANG Yang-yong，
FANG Zhi-yuan

Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences， Beijing 100081， China

Received:2013-03-06 Online:2013-04-09 Published:2013-05-03

甘蓝一代杂种S 单元型的分布

苗雯雯　田　磊　庄　木*　刘玉梅　杨丽梅　张扬勇　方智远

中国农业科学院蔬菜花卉研究所，北京 100081

通讯作者: 庄木，研究员，硕士生导师，专业方向：甘蓝遗传育种及生物技术，E-mail： zhuangmu@caas.cn
作者简介:苗雯雯，硕士研究生，专业方向：蔬菜遗传育种，E-mail：miaowenwen2009@yahoo.cn
基金资助:
中央级公益性科研院所基本科研业务费专项（ICS，CAAS）（1610032011011），农业部大宗蔬菜产业技术体系项目
（hycytx-35），农业部园艺作物遗传改良重点开放实验室项目

Abstract

Abstract: DNA fragments corresponding to kinase domain of S locus kinase（ SRK） gene were
amplified by PCR with 3 pairs of specific primers in all of the 23 cabbage（Brassica oleracea L. var.
capitata L.） F₁hybrids．The S haplotypes of cabbage F₁ hybrid were preliminary identified via DNA
sequencing and Blast analysis．The results indicated that 11 S haplotypes were found in cabbage F₁hybrid，including class Ⅰ haplotypes，S7，S16，S28，S35，S45，S57，S68 and an unknown one
named Sn，and class Ⅱ haplotypes S2，S5 and S15．The highest frequency of S haplotyes was S5 with
19.6% and followed by S28 with 17.4% in this study．Compared over 50 S haplotypes in Brassica，the
11 S haplotypes in cabbage F₁ hybrid suggests that it is very necessary for exploiting more S haplotypes
in cabbage breeding programs.

Key words: Cabbage, S haplotype, F₁ hybrid, Distribution

摘要： 利用SRK 基因激酶区的3 对特异性引物，对23 份甘蓝一代杂种进行PCR 扩增，经过克隆测
序、Blast 分析初步鉴定甘蓝一代杂种的S 单元型。结果表明：供试23 份材料中共出现了11 个S 单元型，
分别是S7、S16、S28、S35、S45、S57、S68 和未知的Sn 等8 个Ⅰ类S 单元型及S2、S5、S15 等3 个Ⅱ
类S 单元型。其中，Ⅱ类S 单元型的S5 出现频率最高，达到19.6%；其次为Ⅰ类S 单元型的S28，达到
17.4%。相比甘蓝类作物中存在的50 多个S 单元型而言，本试验材料涉及的S 单元型并不多，预示在遗传
育种中利用更多的S 单元型是十分必要的。

关键词: 甘蓝, S 单元型, 一代杂种, 分布

CLC Number:

S635.1

MIAO Wen-Wen, TIAN Lei, ZHUANG Mu, LIU Yu-Mei, YANG Li-Mei, ZHANG Yang-Yong, FANG Zhi-Yuan. Distribution of S-haplotypes in Cabbage F₁ Hybrid[J]. , 2013, 1(10): 23-28.

苗雯雯, 田磊, 庄木, 刘玉梅, 杨丽梅, 张扬勇, 方智远. 甘蓝一代杂种S 单元型的分布[J]. 中国蔬菜, 2013, 1(10): 23-28.

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Distribution of S-haplotypes in Cabbage F₁ Hybrid

甘蓝一代杂种S 单元型的分布

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