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Overview of Identification Techniques for Pythium Species Based on Morphology and Molecular Biology |
YIN Ling1,TIAN Xun1,HUO Wan-xue2 |
1College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia, China;2College of Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia, China |
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Abstract Pythium are worldwide distributed oomycetes.In recent years,the plant diseases caused by Pythium become more and more obvious,the area has expanded year by year.And Pythium now regarded as a major harmful genera of plant pathogens.This paper has reviewed the identification techniques for Pythium species based on morphology and molecular biology. It also puts forward an important strategy for disease prevention and control. This strategy takes the conventional morphology as base,uses rDNA-ITS sequences,β-tubulin and CoxⅡ gene,RFLP,RAPD,AFLP and SSR,etc. molecular markers to establish a rapid and accurate diagnosis t echnology.
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Received: 19 March 2013
Published: 19 April 2013
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