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| Genetic Diversity and Fingerprint Based on SCoT Markers in Brassica juncea （L.） Czern. et Coss. |
| LIN Qing1，2，LONG Zhi-jian1，HAN Guo-hui2，HE Qiao1，XIANG Su-qiong1，FAN Yong-hong3，
TANG Jia-jia1，LIANG Guo-lu1* |
| 1College of Horticultural and Landscape Architecture， Southwest University， Chongqing 400716， China；2Chongqing Academy of Agricultural Sciences， Chongqing City Key Laboratory of Adversity Agriculture， Chongqing 401329， China； 3Fuling District Agricultural Science Institute of Chongqing City， Chongqing 408000， China |
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Abstract SCoT markers were used to analyze genetic diversity of 46 mustard accessions．21 primers were screened from 70 SCoT primers and 200 SCoT bands were obtained，including 140 polymorphic bands，with a polymorphism rate of 70%．The genetic similarity coefficient values among these accessions were ranged between 0.77 to 0.95，and the average Nei’s s gene diversity（ H），Shannon’s information index（ I） and Polymorphism information content（ PIC） were 0.198，0.301 and 0.822 7，respectively.The cluster analysis conducted with UPGMA showed that 46 mustard accessions were divided into 2 groups in D1=0.780，and the group Ⅱ were divided into 5 subgroups in D2=0.807．Besides，a SCoT fingerprint of 46 mustard accessions was established with 16 sites of the 140 amplified polymorphic bands．The results showed that 46 mustard accessions can be identified by SCoT fingerprint performed with primers SP4，SP19，SP20，SP23 and SP30.
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Received: 20 February 2013
Published: 25 April 2013
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