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QTL Analysis of Fruits Related Traits in Melon(Cucumis melo L.) |
ZHANG Xue-jiao,GAO Peng,LUAN Fei-shi * |
(College of Horticulture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China) |
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Abstract A recombinant inbred lines(RILs) mapping population of melon(Cucumis melo L.)
comprising 124 F6:7 families was developed from crossing the thick-skin netted melon line WI998
as female parent and thin-skin melon line 3-2-2 as male parent through single-seed descent. This
RILs population was used to construct a linkage map with microsatellite( simple sequence repeat,
SSR) markers.Screening from 608 SSR markers 150 pairs of primers(24.67%)were detected
to be polymorplic between the 2 parental lines.This map contained 17 linkage groups spanning
1 246.67 cM with a mean marker interval of 9.59 cM.Composite interval mapping method was
used to detect QTLs of traits involved in melon fruit:fruit weight( Fw),fruit firmness( Ff),fruit
length( Fl),flesh thickness( Ft) and fruit shape index( Fsi).Total 15 QTLs of fruit were detected
and mapped on linkage groups 1,4,5,6,8,10,13,14,15,17,respectively. Among them,
8 QTLs had contribution rate over 10%. QTL Ff13.2 located at the 13th linkage group had the largest
contribution rate 26.45%.Marker ECM87 was located in the same site with Fsi8.2,and marker
CM33 was close linkage with Fsi15.1,the genetic distance is 0.6 cM.
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Received: 26 February 2013
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