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| Identification of Pathogen of Cowpea Ring Spot and Its Biological Characteristics by Liaoning Province |
| LIU Zhi-heng，LI Jian-bing，AN Xin，TANG Shuang-shuang，ZHENG Chuan ，WANG Shi-wei |
| （Plant Protection College， Shenyang Agricultural University， Shenyang 110866， Liaoning， China） |
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Abstract The newly discovered cowpea disease in Liaoning Province—cowpea ring spot’s
paroxysm symptom was described and its pathogen was identified in this paper．The pathogen biological
characteristics was also preliminary determined. The results showed that through experiment of Koch’s
rule disease test，identification by morphology and molecular biology technology，and comparison with
sequence of Corynespora cassiicola（Berk and Curt.） in GenBank，we found that the homology was up
to 99%．Therefore，the pathogen of cowpea ring spot was C. cassiicola（Berk and Curt）. The results
of biological characteristics determination showed that cowpea leaf juice was the best medium for mycelia
growth．Mannitol and beef extract were the best carbon and nitrogen sources．The optimum temperature
was 28 ℃，and the optimal pH value was 8-10．The most beneficial medium for conidial production
was cowpea seed juice. The best carbon source was maltose and the best nitrogen source was beef extract．
The optimum temperature was 28 ℃，and the optimal pH value was 9．The optimum temperature for
conidial germination was 28 ℃ and the optimal pH value was 7-10．The lethal temperature for spore
was 60 ℃ for 10 minutes，and that for mycelia was 65 ℃ for 10 mi nutes.
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Received: 02 May 2013
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