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| Studies on Classification and Identification based on Morphological Markers and SSR Markers for Elite Varieties of Cucumis melo L. |
| LI Rui-feng，GAO Peng，ZHU Zi-cheng，LUAN Fei-shi* |
| （College of Horticulture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China） |
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Abstract Abstract：Studies on classification and identification of 60 melon（Cucumis melo L.）materials were conducted by SSR marker and Morphological marker. Based on morphological cluster analysis the genetic similarity coefficient of the 60 materials ranged from 0.73-0.94，and at the genetic similarity coefficient of 0.74 these melons could be classified into 2 categories：Cucumis melo ssp. conomon and C. melo ssp. melo. Cucumis melo ssp. conomon could be subdivided into 4 groups，which are preliminarily classified by single fruit weight，peel color，aroma，maturity. The result of SSR cluster analysis showed that the genetic similarity coefficient of the 60 accessions ranged from 0.75-0.95. Also，the test materials could be divided into 6 categories at the genetic similarity coefficient of 0.77. 18 pairs of the SSR primers could detect 4-11 polymorphic bands with an average of 8 bands. The average polymorphism information content（PIC）was 0.71 with the changing range of 0.58-0.88. The fingerprinting effectively distinguished the 60 materials and every accession had unique fingerprinting using QR code.
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Received: 06 January 2014
Published: 14 March 2014
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