Abstract:

Super BSA was performed to screen SNP between 2 mixed DNA pools，which contained 100 pink tomatoes and 100 red tomatoes．SNPs of the tested result were analyzed．The result indicated that 6 SNPs among 127 SNPs on chromosome 1 were highly correlated with the color of tomato fruit，and 1 SNP was closely linked to pink tomato fruit．By this SNP，a polymorphism dCAPS was developed and showed well correlation with red，pink and heterozygous genotypes．It is recently reported that a 603 bp deletion was characterized at the upstream of SIMYB12 gene in pink tomato．Using this deletion，a InDel marker was developed and also showed good polymorphism among red，pink and heterozygous genotypes．These 2 newly developed molecular markers
were further used to analyze genetically the F₂ population．The result was in line with the field observation．Their accordance rate was above 95%，which suggested that these 2 markers can be used for molecular marker assisted selection in tomato breeding．

Key words: Tomato, Pink fruit, dCAPS, InDel, Molecular marker

摘要：

采用Super BSA 方法分别构建100 份粉红色番茄材料和100 份红色番茄材料的DNA 混合池，对测序结果的SNP 位点进行分析。结果表明：在1 号染色体上的127 个SNP 位点中，有6 个与番茄果实颜色高度相关，其中1 个位点与粉红色果实高度相关。利用该SNP 位点开发了1 个共显性dCAPS 标记，在红色、粉红色和杂合F₁ 3 种基因型之间的多态性较好。同时，利用番茄重测序数据分析番茄起源时发现，在粉红色番茄中SIMYB12 基因的上游存在1 个603 bp 的缺失，利用该缺
失突变位点开发了1 个共显性InDel 标记，在红色、粉红色和杂合F₁ 3 种基因型之间也表现出较好的多态性。利用新开发的2 个标记对F₂ 群体进行遗传分析，鉴定结果与田间表型观察结果符合度均达到95% 以上，表明这2 个标记可以用于番茄苗期分子标记辅助选择。

关键词: 番茄, 粉红色果实, dCAPS, InDel, 分子标记