China Vegetables ›› 2016, Vol. 1 ›› Issue (1): 60-63.
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ZENG Feng,HUANG Yong-hong,LING Jian,MAO Zhen-chuan,XIE Bing-yan,YANG Yu-hong*
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曾 凤,黄永红,凌 键,茆振川,谢丙炎,杨宇红 *
基金资助:
国家公益性行业(农业)科研专项(201303014),现代农业产业技术体系建设专项(CARS-25-B-01),中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS),农业部园艺作物生物学与种质创制重点实验室项目
Abstract:
This paper studied the inhibition role of water extracts from Allium tuberosum on Fusarium oxysporum f. sp. conglutinans(Foc)and control effect of pot cultivation by combining in vitro antibacterial assay with potted plant measurement. The result indicated that extracts could clearly suppress mycelia growth and spore germination.The inhibition rate was up to 100% when the concentration of waterextracts reached 0.75 g·mL-1. The spore germination inhibition rate reached 95.47% when the added leek extract concentration came to 0.25 g·mL-1 and was deal with 20 hours. In pot experiments, the disease control efficiency can reach 40%,and mortality fell by 27% 15 days after the treatment when the concentration of waterextracts reached 0.086 g·g-1 in the culture substrate.
Key words: Allium tuberosum, Water extract, Fusarium oxysporum f. sp. conglutinans, Potted plant
摘要:
采用离体抑菌测定和盆栽测定相结合的方法,研究了韭菜水提液对甘蓝枯萎病菌 1 号生理小种的抑制作用和盆栽防治效果。结果表明:韭菜水提液对甘蓝枯萎病菌的菌丝生长速率以及孢子萌发率具有明显的抑制作用,当韭菜水提液浓度为 0.75 g·mL-1 时,菌丝生长抑制率达到 100%;当韭菜水提液浓度为 0.25 g·mL-1,处理 20 h 后观察,孢子萌发抑制率达95.47%;在盆栽试验中,基质中加入韭菜水提液浓度为 0.086 g·g-1 接种 15 d 后,甘蓝枯萎病的防治效率可达 40%,甘蓝死亡率下降 27%。
关键词: 韭菜, 水提液, 甘蓝枯萎病菌, 盆栽试验, 抑制率
ZENG Feng,HUANG Yong-hong,LING Jian,MAO Zhen-chuan,XIE Bing-yan,YANG Yu-hong*. Primary Studies on Inhibitory Effect of Water Extracts from Allium tuberosum on Fusarium oxysporum f. sp. conglutinans in Cabbage[J]. China Vegetables, 2016, 1(1): 60-63.
曾 凤,黄永红,凌 键,茆振川,谢丙炎,杨宇红 *. 韭菜水提液对甘蓝枯萎病菌抑制作用初探[J]. 中国蔬菜, 2016, 1(1): 60-63.
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