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| A Rapid DNA Extraction Method for Large Squash Population |
| LI Wei 1，2，DAI Zu-yun 1，XIA Wei-wei 1，WANG Wen-wen 1 |
1 Anhui Jianghuai Horticulture Seed Co. Ltd.，Hefei 230031，Anhui，China；2 College of Horticulture，Anhui Agricultural
University，Hefei 230061，Anhui，China |
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Abstract Genotype identification is an important link in molecular-marker-assisted breeding and genetic
analysis of gene function．Since this method is used more and more frequently，researchers need a high-throughput
technique．This experiment took a pair of SSR primers of complementary type as the method，and compared
the effect of extracting DNA from cotyledon and root during seedling stage．The effect of extracting DNA from each
tissue of the plant by NaOH-boiling method during maturing stage was also verified．The results indicated that
extracting DNA from root with NaOH-boiling method was a rapid way to measure seed purity．Therefore，extracting
DNA with NaOH-boiling method from all tissues of the matured plant could be used for screening plant genotype．
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Received: 04 February 2017
Published: 03 July 2017
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