Using DAS-ELISA and RT-PCR methods，this study identified pepper pathogenetic virus in experimental fields of Vegetable Insititute，Gansu Academy of Agricultural Sciences. The results showed that in 72 disease infected samples，the total TMV detection rate was 66.7%，whereas CMV had not been detected
as positive. Three pairs of primers were designed to amplify positive samples. A strip with similar segments was amplified only from primer TMV1，which had 97% sequence similarity with ToMMV by BLASTN comparison.Aiming at ToMMV，3 pairs of primers ToMMV1，ToMMVcp and ToMMVspe were designed to amplify ToMMV.A single target band with same size as expected was amplified. Its sequence was 98% accorded with ToMMV by sequencing，proving the virus found in the experimental fields of Vegetable Insititute was truly ToMMV.