Taking artificial pollination as the contrast，an experiment of wall bee pollination with threeline seed production system of pepper was conducted．The results showed that the optimum proportion of male and female parents is 1∶3 with interval cultivation．Wall bee had good adaptability in greenhouse．The fruit setting rate，fruit number of single plant，1 000-grain weight and yield of seed production by wall bee pollination were higher than that by artificial pollination．Thus，the cost of seed production was reduced by a large margin．The cost of each greenhouse was reduced by 86.7%．The application of wall bee pollination in three-line seed production system of pepper is feasible，and the effect is good．