In order to screen a simple，rapid and effective method for inoculation of tomato bacterial canker，this paper compared the effects of 4 different inoculation methods including top leaf shearing，needle penetrating，root soaking and bacterial-soil mixing methods on tomato canker occurance degree. The results showed that top leaf shearing was the best inoculation method. Its incidence rate was 100%，and disease index was over 90. This method is simple to operate，applicable for evaluating the pathogenicity of bacterial and fungicide control effect to tomato bacterial canker. Through pot experiment in greenhouse，this paper compared the control effects of 23 microbial agents for tomato bacteria canker and screened 3 safe and highly efficient microbial agents，naming bacillus IVF 018，bacillus IVF 021 and bacillus IVF020. Their control effects were all over 98%.