Abstract:

Tomato yellow leaf curl virus（TYLCV）is an etiology of Tomato yellow leaf curl disease
（TYLCD），which will cause serious damages to tomato production．It is of important significance to exploit rapid
and accurate detection method for controlling this disease．According to DNA-A gene sequence of TYLCV，this
study designed special RPA primers and established a method named TYLCV-RPA，and analyzed the specificity
and sensitivity of this method．The results indicated that TYLCV-RPA method could amplify specific band of 343
bp from sample tomatoes with positive TYLCV．The reaction condition was 37 ℃ for 40 min．This method needed
short time period for amplification with low requirement for equipment．It had no cross reaction with the other
viruses and had good specificity．Its sensitivity was 10 times of the PCR method．It is suitable for rapid detection
of TYLCV．

Key words: Tomato yellow leaf curl virus, Recombinase polymerase amplification, Isothermal amplification, Detection method

摘要：

番茄黄化曲叶病毒（Tomato yellow leaf curl virus，TYLCV）是引起番茄黄化曲叶病毒病的病原，在生产中造成严重危
害，开发快速准确的检测方法对该病害的防控具有重要意义。依据番茄黄化曲叶病毒DNA-A 基因序列，设计用于重组酶聚
合酶等温扩增（RPA）检测的特异性引物，建立了TYLCV 的重组酶聚合酶等温扩增检测方法，并分析该方法的特异性和灵
敏度。结果表明，建立的TYLCV-RPA 方法可从TYLCV 阳性的番茄样品中扩增出343 bp 的特异性条带，反应条件为37 ℃
恒温下40 min，扩增时间短，对设备要求低，且与番茄其他病毒无交叉反应，特异性好，灵敏度可达到PCR 方法的10 倍，
适用于TYLCV 的快速检测。

关键词: 番茄黄化曲叶病毒, 重组酶聚合酶扩增, 等温扩增, 检测方法