Abstract:

In order to screen and obtain good bio-control bacterial strain，this study isolated and screened
cultivable bacteria from the top paddy soil by means of dilution method and inhibition test in plate. An effective
antagonistic bacteria named LRS-1 was isolated from 167 cultivable bacteria strains. It was identified as
Paenibacillus polymyxa bacteria. The strain owned as much as 68% inhibition rate against Phytophthora capsici
in door and 63.4% controlling effect on Phytophthora blight of pepper in greenhouse. The result of Real-time
PCR test indicated that this strain had remarkable inhibitory effect on Phytophthora pathogen population in
rhizosphere.

Key words: Phytophthora capsici, Antagonistic bacteria, Screen, Identification, Control effect

摘要：

以筛选、获取优良辣椒疫霉病害生防菌株材料为目标，通过平板稀释法与对峙法对水稻土壤耕层可培养细菌进行
分离和筛选，成功从167 株可培养细菌中分离、筛选获得1 株拮抗菌LRS-1，并被鉴定为多粘类芽孢杆菌（Paenibacillus
polymyxa）。该拮抗菌对辣椒疫霉菌具有68% 左右的室内持久抑菌率以及63.4% 的温室病害防效；Realtime-PCR 检测结果表
明该拮抗菌对根围疫霉菌侵染数量具有显著的抑制效果。

关键词: 辣椒疫霉, 拮抗菌, 筛选, 鉴定, 防效