Abstract:

Leaf angle is an important part of plant type，which affecting crop photosynthetic efficiency and yield．Taking ‘Heinz1706’with open and flat leaves as female parent and‘161-817’with upthrow leaves as male parent，this study constructed F_2：3 populations to conduct fine mapping of tomato leaf angle gene．Results of genetic analysis revealed that the leaf upthrow to open and flat leaf was shown partially dominant．The results of combining BSA method with QTL mapping indicated that there were 2 major QTL （LA1-1 and LA1-2）existed on chromosome of tomato No.10 and were connected with tomato leaf angle phenotype．Among them，LA1-1 was located between molecular markers A-10 and A-5 with 18.7% genetic contribution rate and 1.58 Mb physical distance．There were 210 genes encoded in this region．LA1-2 was located between molecular markers B-3 and B-9．The genetic contribution rates was 22.5% and physical distance was 378 kb．There were 32 genes encoded in this region．

Key words: Tomato, Plant type, Leaf angle, Exquisite location

摘要：

叶夹角作为株型的重要组成部分，是影响作物光能利用率和产量的重要因素。以叶片上举的161-817 为父本，叶片平展的Heinz1706 为母本构建F_2∶3 群体，对番茄叶夹角基因进行精细定位。遗传分析结果显示，叶上举对叶平展表现为部分显性。混池分析结合QTL 作图结果显示，番茄10 号染色体上存在2 个主效QTL（LA1-1 和LA1-2）与番茄叶夹角表型有关。其中，LA1-1 位于分子标记A-10 和A-5 之间，遗传贡献率为18.7%，物理距离为1.58 Mb，该区域编码210 个基因；LA1-2位于分子标记B-3 和B-9 之间，遗传贡献率为22.5%，物理距离为378 kb，该区域编码32 个基因。

关键词: 番茄, 株型, 叶夹角, 精细定位