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Fine Mapping of Tomato Leaf Angle Genes |
LIU Xing-yu,GUO Yan-mei,SU Xiao-mei,HUANG Ze-jun,WANG Xiao-xuan,DU Yong-chen,GAO Jian-chang* |
Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing100081,China |
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Abstract Leaf angle is an important part of plant type,which affecting crop photosynthetic efficiency and yield.Taking ‘Heinz1706’with open and flat leaves as female parent and‘161-817’with upthrow leaves as male parent,this study constructed F2:3 populations to conduct fine mapping of tomato leaf angle gene.Results of genetic analysis revealed that the leaf upthrow to open and flat leaf was shown partially dominant.The results of combining BSA method with QTL mapping indicated that there were 2 major QTL (LA1-1 and LA1-2)existed on chromosome of tomato No.10 and were connected with tomato leaf angle phenotype.Among them,LA1-1 was located between molecular markers A-10 and A-5 with 18.7% genetic contribution rate and 1.58 Mb physical distance.There were 210 genes encoded in this region.LA1-2 was located between molecular markers B-3 and B-9.The genetic contribution rates was 22.5% and physical distance was 378 kb.There were 32 genes encoded in this region.
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Received: 05 June 2019
Published: 02 September 2019
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