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| Establishment of a Regeneration System in Wax Gourd（ Benincasa hispida Cogn.） |
| LI Zhifang1，YAN Jinqiang2，HAN Xiangfeng1，XIE Dasen2，LIU Wenrui2，WU Qianhui1，JIANG Biao2* |
1Foshan Institute of Agricultural Sciences，Foshan 528145，Guangdong，China；2Vegetable Research Institute，Guangdong
Academy of Agricultural Sciences，Guangzhou 510640，Guangdong，China |
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Abstract In this study，taking cotyledon nodes as explants，the effects of different proportions and
concentrations of hormones and days of dark culture on cotyledon node callus-induced regeneration buds were
optimized，the effect of different concentrations of hormone combinations on adventitious bud proliferation
were studied，and the rooting and transplanting of regenerated seedlings were tested，finally we established
a relatively comprehensive set of regeneration system of wax gourd cotyledon nodes．According to the result
of orthogonal experiment，the optimum combination for inducing adventitious bud differentiation was MS +
2，4-D 0.1 mg · L-1 + IBA 0.10 mg · L-1 + 6-BA 0.8 mg · L-1 after 7 days dark culture；and the time of dark
culture and concentration of 2，4-D were major factors affecting regeneration．The best medium for proliferation
of adventitious bud was MS + NAA 0.05 mg · L-1 + 6-BA 0.2 mg · L-1，with a proliferation coefficient of 2.487．
The rooting rate of the regenerated seedlings on the rooting medium was 100% on 1/2MS + NAA 0.2 mg · L-1
and the survival rate of the domesticated seedlings was more than 80% after transplanting．
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Received: 15 September 2020
Published: 18 December 2020
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